Small benchtop flow cytometers from stratedigm, inc. Nowash, nolyse sample prep options with clogresistant fluidics. Flow cytometer the cytoflex flow cytometer is designed to deliver superior performance, flexibility, and ease of operation for research applications. Explore the attune nxt flow cytometer and autosampler. If you use cytospec, please acknowledge it in the following way. The bd accuri c6 plus personal flow cytometer is the newest generation of the bd accuri platform. This version of attune nxt software for windows 10 computers supports compliance with 21 cfr part 11 fda guidelines for security, auditing, and. The attune nxt flow cytometer includes a dongle and 1 license for the attune nxt software. Description the attune performance tracking beads are designed for use with attune cytometric software and attune nxt software to automatically characterize, track, and report the performance of the attune acoustic focusing cytometer and attune nxt acoustic focusing cytometer daily and over time. Catalog of free flow cytometry software the original cytometry software catalog was developed and managed by dr. Fcsalyzer free program for analysing flow cytometry data. Volume 3 jolene bradford, associate director flow cytometry, speaks about the software of the new attune nxt flow cytometer.
When you open this page and download the software, you are agreeing not to reverseengineer or incorporate this software into any commercial product. The attune nxt flow cytometer system was developed with the goal of removing barriers associated with flow cytometry and enabling new research. Flow cytometry is used in research and clinical laboratories for many applications including chromosome analysis, cancer diagnosis, protein. Since its initial unveiling, the compact system with innovative acoustic technology is moving ahead with added new functionalities and capabilities making multiparametric flow. Purchase a stratedigm benchtop flow cytometer and be confident that you have made the best possible flow cytometry investment. By controlling all steps in highthroughput flow cytometry, users can quickly and easily create multiplate experiments, acquire data, and perform indepth analysis on thousands of samples all in one software platform. Designed for all types of research, this instrument fits into any workflow from cell counts to screens for multicolor flow cytometric endpoint analysis. In the global flow cytometry market, the company offers a variety of analytical instruments and laboratory equipment, software, services, and consumables. To learn more about how to set and monitor optimal voltages for a flow cytometry experiment, and to get access to all of our advanced materials including 20 training videos, presentations, workbooks, and private group membership, get on the flow cytometry mastery class wait list. The attune nxt flow cytometer offers customizable configurations and can be set up with the attune nxt autosampler and integrated robotics. The 2019 attune nxt flow cytometer continues to offer more options, increased flexibility, and software updates that further improve performance, reliability, and robustness. If a hard reboot is necessary, turn off the software first, then the attune nxt cytometer, then the autosampler.
Flow cytometry within reach the analytical power and versatility of todays laserbased flow cytometry systems have unlocked the mysteries of cell biology and empowered entirely new fields of research. The functions of the attune nxt acoustic focusing cytometer are controlled by the attune nxt software. Attune acoustic focusing flow cytometer from thermo. Hot spots throughout the tool feature data, researcher opinions, and technical specifications. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. The attune nxt flow cytometer was developed with the goal of removing barriers associated with flow cytometry and enabling new research. Mouse operation enables rotation, zoom, and measurement. The guava pca96 is a fully automated flow cytometer built for accurately count cells while increasing production within your lab. Martz, the site will now be managed by the purdue university cytometry laboratories. How does attune cytometer differ from conventional flow cytometers. The attune nxt flow cytometer, designed using acousticassisted hydrodynamic focusing, achieves samplethroughput rates of of 12. I am currently using a very old flow cytometer and having an issue with its software.
The attune nxt flow cytometer enables you to conduct experiments more responsibly, while generating less waste. Attune nxt flow cytometer system combines precision with performance in a true benchtop flow cytometer, with up to 4 lasers and 16 parameters of detection. The essence of a flow cytometer is that cells are stained with a fluorescent dna dye acridine orange and then forced through a glass channel in a liquid suspension. Fact sheet attune nxt flow cytometer the attune nxt. High quality the attune shutdown solution is prepared, filtered, and packaged in a sterile environment to ensure the highest quality. Invitrogen attune nxt flow cytometry software offers powerful, userdefined experimental analysis via an intuitive and easytouse interface.
Youd expect all that from any new flow cytometer but the attune. The attune nxt flow cytometer combines precision with performance in a true benchtop flow cytometer with up to 4 lasers and 16 parameters of detection. The attune nxt flow cytometer is different due to the acoustic field in the hydrodynamic system, as it reduces the dependence on the fluid to push the cells into an aligned core stream. The instrument also conforms to the ul and cancsa general requirements 61010. The attune nxt flow cytometer is different due to the. Turn on in reverse, allowing 30 seconds between each step i. Selecting the optimal cytometer for your experiment can be as important as the panel you design. An introduction to compensation for multicolor assays on digital flow cytometers bd biosciences august 2009 practices to avoid. With the option to be configured with up to 4 lasers and 14 colors, the attune nxt acoustic focusing cytometer was designed as a modular system to fit most experimental design needs and lab budgets. However, you should also consult your flow core manager for their recommendation. Attune nxt flow cytometer thermofisher top row shows attune nxt data analyzed with system software. Which benchtop flow cytometer is good attune acoustic focusing cytometer or merck millipore easycyte 62l.
It is programmed in java and should therefore work on many different operating systems. The software plots the data in a very set way, but i. The readytouse 1x shutdown solution was designed to eliminate the need for dilution prior to use, as well as access to a purified water system. The attune nxt software may be downloaded onto more than one computer, however the software will only run on computers that also have a dongle inserted. Bottom row shows the same plots generated within kaluza after importing the fcs file. This includes bringing the power of flow cytometry to the analysis of more sample types, including cancer cells. I am trying to find a free software that can analysis fcs files obtained by attune nxt flow cytometer. Used flow cytometers bd facsaria, facscanto and accuri c6. Sop for lsr ii flow cytometer fhhvtne0022 page 2 of 18 version 6. Stratedigms automation suite is designed based on the same principles as our flow cytometers innovative, compact, and flexible. Attune nxt flow cytometer software thermo fisher scientific. Bd accuri c6 software has an intuitive user interface designed with the researcher in mind. Fcsalyzer is a free program for analysing flow cytometry data.
We have compiled this useful list of popular cytometers for your reference. D etection of platelets in whole blood using the attune. But it does not recognize my fcs files obtained by attune nxt flow cytometer. Attune meet the inventor series volume 4 jolene bradford, associate director flow cytometry, speaks about the fluidic design of the new attune nxt flow cytometer. Sop for lsr ii flow cytometer university of washington.
Since its initial unveiling, the compact system with innovative acoustic technology is moving ahead with added new functionalities and capabilities making multiparametric. Attune shutdown solution 1x 250ml flow cytometry supplies. Attune nxt flow cytometer with attune nxt autosampler. Recently we had the attune nxt cytometer in the lab for a demo. Thermo fisher scientific certifies that the attune nxt flow cytometer conforms to relevant directives to bear the ce mark. Of course, this overview merely scratches the surface of the intricacies of a flow cytometer. Select your lasers, colors, automation, service contract, and more. Attune nxt flow cytometer from thermo fisher scientific.
Ive recently started doing flow cytometry bd accuri c6 in a core facility at my university, but my lab doesnt have any softtware to analyze the data. However, knowing these simple basics will allow you to approach almost any instrument on the market and have some knowledge of whats going on inside the box. Feel at ease with the clog resistant, walkaway automationplate autosampler. Attune nxt flow cytometer thermo fisher scientific. Attune nxt flow cytometer continues to offer more options, increased flexibility, and software updates that further improve performance, reliability, and robustness. Attune nxt flow cytometer quick maintenance and troubleshooting guide the invitrogen attune nxt flow cytometer is designed to require minimal maintenance. Flow cytometer automation stratedigm flow cytometers. Attune software for the attune acoustic focusing cytometer and. The invitrogen attune nxt flow cytometer was developed with acousticassisted hydrodynamic focusing technology, and advanced fluidics designed to minimize clogging and effectively handle a broad range of cell types.
Configure your futureproof flow cytometer stratedigm. Flow cytometry is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles in this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. Catalog of free flow cytometry software purdue university. Special download site for pucl software purdue university. Flow cytometry software the ric facility offers many different ways for investigators to analyze their flow cytometry data. Applied biosystems attune acoustic focusing cytometer auto sampler upgrade kit 1 each flow cytometer accessories flow cytometry.
Flow cytometry fcm is a technique used to detect and measure physical and chemical characteristics of a population of cells or particles in this process, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. When using a flow cytometer, a sample containing cells or particles is suspended in a fluid and injected into the flow cytometer instrument. The attune nxt flow cytometer is a class i laser product per center for devices and radiological health cdrh regulations and eniec 60825. Attune performance tracking beads 3ml flow cytometry. The bd accuri c6 is a personal flow cytometer that brings cell analysis within reach by being easy to use, simple to maintain, and affordable. The demo model had blue, yellow, red and violet lasers. The attune nxt flow cytometer conserves fluids with. Scope this sop applies to the use of the lsr ii flow cytometer within the fhcrc hvtn endpoint assay laboratory. An introduction to compensation for multicolor assays on.
Fcsalyzer offers an easy whatyouseeiswhatyou get interface and provides the standard analysing tools dot plots, histograms, complex gating strategies and associated statistics. Precise alignment, even at high sample rates up to 1 mlmin will be maintained. Analysis can be performed on the bd accuri c6 cytometer or can be exported into thirdparty programs such as fcs express. The precision of flow cytometry, coupled with the precision biochemistry of the scsa test, provides unparalleled measures of sperm chromatin and dna integrity. Fact sheet attune nxt flow cytometer the attune nxt flow. Mar, 2019 with acousticassisted hydrodynamic focusing, the attune nxt flow cytometer is designed to deliver both excellent data quality and higher sample rates by uncoupling cell alignment from sheath flow. Flow cytometers resources flow cytometry antibodies. Build your perfect flow cytometer with stratedigms configure your product. Flow cytometry gui for matlab file exchange matlab central. Measurement of microsphere concentration using a flow. The tabbed interface provides quick access to the collection, analysis, and statistics functions. Attune acoustic focusing cytometer user guide fresno state.
Your custom configuration form will allow stratedigm to price your instrument and provide you with a quote. The attune acoustic focusing flow cytometer delivers higher sensitivity when you needed most. Which benchtop flow cytometer is good attune acoustic. Designed for faster experimental run times, the invitrogen attune nxt compact flow cytometer offers rapid and accurate analysis for. Lminute, the instrument operates predominantly as a hydrodynamic focusing instrument. Any free flow cytometry software can import attune nxt fcs file. The dscatter function is insanely fast and well written. The default threshold channel for most commercial flow cytometers, including the attune nxt flow cytometer, is fsc. Best of all, the software is free with unlimited copies so you can analyze your flow. Applied biosystems attune acoustic focusing cytometer auto. The attune nxt flow cytometer offers increased flexibility, intuitive software, and a high throughput 96 well platform for data acquisition with hydrodynamic focusing technology. This version of attune nxt software for windows 10 computers supports compliance with 21 cfr part 11 fda guidelines for security, auditing, and electronic signatures using an sae console. Guava pca96 flow cytometer with computer and software.
Stratedigm offers seamless integration of automation, highthroughput technology, instrumentation, and software that delivers the utmost in productivity. Therefore the measurements with the attune flow cytometer can provide reliable values of the microsphere concentration. Top 5 vendors in the global flow cytometry market from 2016. Additional resources are available on the flow cytometry technical resources page. Flow cytometry is a laser based technology used to count and sort cells, detect biomarkers and engineer proteins, by suspending cells in a stream of fluid and passing them by an electronic detection system. To ensure reliability of the flow cytometer, you must perform basic preventive maintenance procedures on a regular basis, as listed below. Buying a new flow cytometer is a huge investment, and with the extensive variety and diversity of flow cytometers on the market, it is often difficult to ensure you purchase the instrument that. Attune nxt software permits to adjust flow rates, compensation and voltages. Flow cytometry is a powerful technique that can be used to detect and measure the properties of a population of cells or particles.
Simplified system setup, data acquisition, analysis, and export of experimental results are integrated into a complete workflow solution with cytexpert software. Invitrogen attune nxt flow cytometer from thermo fisher. The light source can be a laser, arc lamp or light emitting diode led. Flow cytometry is a technology that is used to analyse the physical and chemical characteristics of particles in a fluid as it passes through at least one laser. The software is preinstalled to the computer workstation supplied with the attune nxt acoustic focusing cytometer, and the attune nxt software icon i.
The attune nxt flow cytometer can be equipped with up to 4 lasers and 16 detection channels. Flow cytometry our interactive and laboratory instruction courses deliver flexible, handson training, from refresher to indepth, to help meet the needs of cellular biology professionals and users of the attune acoustic focusing cytometer. With acousticassisted hydrodynamic focusing, the attune nxt flow cytometer is designed to deliver both excellent data quality and higher sample rates by uncoupling cell alignment from sheath flow. Presented plots and values are for illustrative purposes only and are based on customer reported. The pca96 from guava now owned by millipore has the capability of reading 96. Fluid conservation in conventional flow cytometers, increasing the sample flow rate allows faster data acquisition but lower resolution, and it requires large amounts of fluid. According to the above needed specifications, either the attune nxt from life technologies or the lsr fortessa from bd could be purchased in our situation available. Safety on page 68 for the complete the chemical safety alert words four safety alert words appear in life technolo gies user documentation at points in the document where you need to be aware of relevant hazards. Top 5 vendors in the global flow cytometry market from.
The attune nxt acoustic focusing cytometer has a range of flow rates from 12. The attune software is designed to simplify and speed up. Volume 3 jolene bradford, associate director flow cytometry, speaks about the software of the new. Attune nxt flow cytometer software thermo fisher scientific us. Cytospec software is from purdue university cytometry laboratories and if you wish you. The ze5 cell analyzer is an innovative flow cytometer with flexible configurations to meet a broad range of experimental complexities and throughput needs accessible for novice flow cytometry users yet flexible enough for the most experienced flow cytometry professionals. A software tool for exploring the hardware, software, optics and fluidics of the attune nxt flow cytometer. The common practice of using fsc is largely a consequence of the utility of this channel in excluding small particles, including platelets and debris from white blood cell analysis. The attune nxt flow cytometer was developed with the goal of removing barriers associated with flow cytometry and enabling new science. The page will give the basics of the different applications available as well as their differences so one might be better informed of their available options. The sample is focused to ideally flow one cell at a time through a laser beam, where the light scattered is. Invitrogen attune nxt flow cytometer demo video youtube. The attune can have up to 14 parameters plus forward and side scatter from up to four lasers.
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